dectin 1 pe  (Miltenyi Biotec)

Journal: Journal of Biomedical Science

Article Title: Variants of human DECTIN-1 rs16910526 are linked to differential reactive oxygen species production and susceptibility to tuberculosis

doi: 10.1186/s12929-024-01067-w

Figure Lengend Snippet: Correlation between Dectin-1 expression and ROS index in response to Mtb in PMM from HS and TB patients. PMNs were incubated with DHR and treated with Mtb or left unstimulated for 90 min. Oxidized DHR emission due to ROS generated in the presence of Mtb was then assessed using flow cytometry in the FL-1 channel. The ROS index was calculated as the mean fluorescence intensity (MFI) after Mtb stimulation / MFI after culturing with medium. Dectin-1 expression in fresh peripheral blood PMNs was assessed by labelling with anti-Dectin-1-PE antibodies and the corresponding nonspecific isotype (IgG) using flow cytometry. HS: p < 0.0008, r2 = 0.7316; TB p < 0.0001, r2 = 0.8506 (Spearman test)

Article Snippet: Freshly isolated PMNs were assessed using saturating concentrations of monoclonal mouse anti-human Dectin-1-PE-conjugated antibodies (clone 15E2), along with their corresponding isotype controls (R&D Systems Inc., Minneapolis, USA).

Techniques: Expressing, Incubation, Generated, Flow Cytometry, Fluorescence

Journal: Journal of Biomedical Science

Article Title: Variants of human DECTIN-1 rs16910526 are linked to differential reactive oxygen species production and susceptibility to tuberculosis

doi: 10.1186/s12929-024-01067-w

Figure Lengend Snippet: Mtb -induced ROS production and Dectin-1 expression in PMNs harbouring rs16910526 genotypic variants. A ROS index in the total population (**p < 0.0001); B ROS index in HS and TB patients (A/A versus A/C genotypes **p < 0.0001; A/C in HS vs. TB #p < 0.02); C Representative histogram of DHR emission in samples with A/A and A/C genotypes compared to control (grey line) and unlabelled cells (grey shadow); D Dectin-1 expression in the PMN in the total population (A/A versus A/C genotypes **p < 0.0001); E Dectin-1 expression in HS and TB patients (A/A versus A/C genotypes **p < 0.0001); F A representative histogram of Dectin-1 expression in two different individuals (A/A and A/C ) and the corresponding nonspecific isotype (IgG) labelling (grey shadow). The values are expressed as the mean ± SEM of the MFI determined by flow cytometry. P values were calculated using the Mann–Whitney U test for unpaired and non-parametric samples

Article Snippet: Freshly isolated PMNs were assessed using saturating concentrations of monoclonal mouse anti-human Dectin-1-PE-conjugated antibodies (clone 15E2), along with their corresponding isotype controls (R&D Systems Inc., Minneapolis, USA).

Techniques: Expressing, Control, Flow Cytometry, MANN-WHITNEY